Combining real-time fluorescence spectroscopy and flow cytometry to reveal new insights in DOC and cell characterization of drinking water

Sudden changes in drinking water quality can cause harmful consequences for end users. Thus, real-time monitoring of allow early warning and provide crucial gains securing safe distribution. This study investigated the advantages simultaneous measuring flow cytometry fluorescence spectroscopy. A contamination event was a laboratory-scale analysis by spiking samples with organic nutrients. Flow cytometric data were analyzed creating fingerprints based on differentiation into high low nucleic acid cells (HNA/LNA). The detailed characterization these showed that an increase HNA indicated bacterial growth potential even before actual TCC increases. decomposed via PARAFAC method to reveal seven fluorescent components. Three aromatic protein-like components associated microbiological condition cells; namely, Components 4 ( ? Ex = 279 nm, Em 351 nm), 6 332 7 276 302 nm). Component identified as possible variable appropriate TCC, whereas compounds representing nutrients organisms present water. Overall, combining both methods be powerful tool guarantee quality.